University of Wisconsin - Stout

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UV/VIS Diode Array Spectrophotometer

This spectrophotometer is designed to measure the amount of light absorbed at each wavelength of the ultraviolet and visible light regions.  The absorbance can then be plotted against the wavelength of light, to give an absorbance spectra.

Electromagnetic radiation in the visible and ultraviolet regions (wavelengths from 200-800nm) can be absorbed by electrons in most molecules. The UV or visible light energy is absorbed when the electrons are excited from their ground state to some higher energy level.